What is the net gain of ATP from one molecule of glucose during glycolysis?

During glycolysis, one molecule of glucose undergoes a series of enzymatic reactions to be converted into two molecules of pyruvate. Throughout this process, a total of 4 ATP molecules are produced; however, 2 ATP molecules are consumed in the initial steps of glycolysis. As a result, the net gain of ATP from one glucose molecule is calculated by considering both the ATP produced and the ATP used. Thus, the net gain is 4 ATP produced minus 2 ATP consumed, which equals 2 ATP. This means that for every molecule of glucose that is processed in glycolysis, the cell has a net gain of 2 ATP molecules, making that the correct answer.